Identifying suitable donors for faecal microbiota transplantation (FMT) is complex, costly, and time-consuming. Here, we present the donor data set for the gut bugs trial. This is published as part of Donor screening outcomes for faecal microbiota transplantation: from recruitment to encapsulation.Brooke C. Wilsona,b,#, Ry Y. Tweedie-Cullena,#, Taygen Edwardsa, Karen S. W. Leonga, Christine Creagha, Marysia Depczynskia, Benjamin B. Alberta,c, José G. B. Derraikd,e, Justin M. O’Sullivana,b,f,g, & Wayne S. Cutfielda,c,a Liggins Institute, University of Auckland, Auckland, New Zealand.b The Maurice Wilkins Centre, University of Auckland, Auckland, New Zealand.c A Better Start – National Science Challenge, University of Auckland, Auckland, New Zealand.d Department of Paediatrics: Child & Youth Health, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealande Environmental-Occupational Health Sciences and Non-Communicable Diseases Research Group, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Thailandf MRC Lifecourse Epidemiology Unit, University of Southampton, University Road, Southampton, UKg Singapore Institute for Clinical Sciences, Agency for Science Technology and Research, Singapore#These authors contributed equally (co-primary).