analysed_reads file contains raw nanopore reads extracted from a nanopore dataset readsf or the Tasmanian devil (NCBI accession PRJEB34649) in FASTA format used to determine rDNA unit size..Con_reads files contain raw nanopore reads individual rDNA unit sequences were extracted from to generate the consensus sequence in FASTA format.Con_indiv_units files contain individual rDNA unit sequences extracted from nanopore reads used to generate the long and short consensus sequence in FASTA format.Consensus_alignment files contains the alignement output in .geneious format.Annotated_Consensus files contains the two annotated consensus sequences in .geneious format.align_Tasmanian_Devil_con_with_ref file contains the rDNA consensus sequence aligned to a reference sequence (NCBI accession GL000220; see Agrawal & Ganley, 2018).Average rDNA unit size was generated using the included excel spreadsheet.